Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETVLEGEFDELMRILKEALEVAGQEADNVFANVKINV--GEILSIDEKLEKYTETTH 2EPI Chain:A ((5-99)) RKVVAEVSIIPLGKGASVSKYVKKAIEVFKKYDLKVETNAMGTVLEGDLDEILKAFKEAHSTVLNDVDRVVSSLKIDERKDKENTIERKLKAIGE---

General information: TITO was launched using: RESULT: Template: 2EPI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 370 -44107 -119.21 -474.27 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -119.21 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_2EPI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EPI-query.scw PDB file : Tito_Scwrl_2EPI.pdb: