Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSTQQWQSVTWFEVDEHQAGQRIDNFLFSRLKGVPKSRIYRLIREGQVRVNKKRIKAETKLAIGDQIRVAPIRYEQKDETAAPVSDSVAQGLLSRVVYEDEGLLVVNKPSGIAVHGGSGVAYGLIEALRAATG-KKYLELIHRIDRDTSGLVMISKKRSTLKLLQDMLREHKIRKTYAAIVKGQVSLDKQLIDAPLFRYELANGERRVRVSKEGKPSKTEWVVAERFKNATLVHASPLSGRTHQIRVHGLSIGHPLVGDDKYGHNTAYT------GPEARRLCLHAMRLDIPGYP-----TIEAPLPEDMTQLLEALRVAK 1V9K Chain:B ((3-227)) -----------------------------------------------------------------------------------------------VIMYEDDHILVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSALRSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSETRFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYG-DREFDRQLTEAGTGLNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKMRNA-

General information: TITO was launched using: RESULT: Template: 1V9K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1105 5969 5.40 28.02 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : 5.40 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_1V9K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V9K-query.scw PDB file : Tito_Scwrl_1V9K.pdb: