Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNHENYWTFCPHNLFWIDRKINRIDMSQTTQTDIITLGDVATRLPSFIPKVPHILNGLKQAYLRTSNTPTGLGIAFEKAVKRNPKGTALLFEDQSYSYEALNEWANQISHYYLSLGARKGDVIAVMIENRSELIATIVGLAKIG-VTIALVNTSQVGKVLAHSINLVKPIAVIAGEEVRAAIDEIRQDLNVPKDRFHWFADQATRQKSGTAPKGYVNLADQIDQFPKFNPSTTRSVTGNDGLFYIYTSGTTGLPKAVIFKHSRWTLAYGTYGHILNLGPDDVMYVTLPLYHATGVVVCWCGVIAGSATLAIRRKYSTSAFWKDVQKFNASAIGYVGELCRYLMDAPVTELDRNHR--------------VTKMIGNG---MRPNIWDKFKQRFGVKEVLELYASSEGNVGFSNIFNFDNTVGFSPTPYAIIQFDKEKNEPIR------DKNGWCQKVKAGEVG-----LLIGKITSRSPFD---------------GYTDPEKNKSVIWKDVFKK------GDSYFNTGDLVR--DIGFR---HAQFVDRLGDTFRWKGENVSTTEVENMVCEYDK-IAEAVVYGVEIPNTNGRAGMAAITLADGEELNDADLTEMVTVFKKCLPTY---AVPVFLRVQKKVETTGTFKYQKNKLKEEAFNPSKTSERLLALLPGASSYCD--ITTEIFDNIQAYKYRF 5EY9 Chain:A ((3-626)) -----------YHNPFIVNGKIR-----FPENTNLVRHVEKWARV-----------RGDKLAYRFLDFSTERDG-----------VERDILWSEFSARNRAVGARLQQVTQ--------PGDRIAILCPQNLDYLISFFGALYSGRIAVPLFDPAEHVGRLHAVLDDCTPSTILTTTDSAEGVRKFIRS-RSAKERPRVIA---------------------VDAVPTEVASTWQQPNELTTAYLQYT-----VPSGVQITHLNLPTNVLQVLNALEGQEGDRGVSWLPFFHDMGLITVLLASVLGHSFTFM----TPAAFVRRPGRWIRELARKPGETGGTFSAAPNFAFEHAAMRGVPRDDEPPLDLSNVKGILNGSEPVSPASMRKFFKAFEPYGLRE--TAVKPSYGLAEATLFVSTTPMDEVP-TVIHVDRDELNKQRFVEVAADAPNAVAQVSAGKVGVDEWAVIVDTETASELPDGQIGEIWLHGNNLGIGYWGKEEESAQTFRNILKSRVPESHAEGAPDDGLWVRTGDYGTYFKGHLYIAGRIKDLVIIDGRNHYPQDLEYTAQESTKALRVGYVAAFSVPANQLPQKVFDDPHAGLSFDPEDTSEQLVIVGERAAGTHKLEYQPIADDIRAAIAVGHGVTVRDVLLVSAGTIPRTSSGKI-GRRACRTAYIDGSLRSTVFA---------

General information: TITO was launched using: RESULT: Template: 5EY9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3162 -50800 -16.07 -92.53 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -16.07 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.270

(partial model without unconserved sides chains): PDB file : Tito_5EY9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EY9-query.scw PDB file : Tito_Scwrl_5EY9.pdb: