Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKASLRLRLDQLCDRHEELTALLADAEVISDNKRFRKLSREHSDLTEITEVWGKYRQAEEDIETAEMMKSDPDFKDMAEEEIQANKALLEELESQLNILMIPKDPNDSNAAYLEIRAGTGGDEAAIFSGDLFRMYSKYAESQGWRIEVLSENEGEHGGFKEVICRVDGDGVYGRLKFESGAHRVQRVPATESQGRVHTSACTVAILPEIDVDTNVEINPADLRIDTYRASGAGGQHINKTDSAVRITHIPTGTVVECQEERSQHKNKAKAMALLVSRLENAKRAAADAATSEMRRDLVGSGDRSERIRTYNYPQGRMTDHRINLTLYKLDAIMEGDLTELLDSLHREYQADQLAMLAQENGG 2RSM Chain:A ((4-111)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GSSGKWGLRLQEKPALLFP-GMAASTVQVAGRKDYPA--LLPLNESELEEQFVKGHGPGGQATNKTSNCVVLKHVPSGIVVKCHQTRSVDQNRKIARKVLQEKVDVFYNSG-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2RSM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 240 -11639 -48.49 -107.76 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -48.49 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_2RSM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RSM-query.scw PDB file : Tito_Scwrl_2RSM.pdb: