Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTELQDIDFVELSDEEMHLYSRQILLDGWDIEAQEKLKLANVLIVGAGGIGCSSAELLARAGVGKITLIDADTIEISNLQRQIAFGHEDIGRYKAEVLAKRLQKINPYICVEYFNERLDEHNIDKLVEHQDVVLDGCDNFTTRYLVNAACKKHQVALISASAIGFQAQMFMVEG--DSACYECLFPKEQHANEGLRCAESGVLATTPVMIASLQAHHTLLYLGLNRTPLKQKLLLWDGLNMTQRIVSFDKDINCPFCQAS 1ZFN Chain:B ((3-246)) -----------MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAALYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTALQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMVLTPPWEQGCYRCLW------------AGVVGPVVG--VMGTLQALEAIKLLSGIETPAGE-LRLFDGKSSQWRSLALRRASGCPVCG--

General information: TITO was launched using: RESULT: Template: 1ZFN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1268 -153664 -121.19 -668.10 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -121.19 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_1ZFN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZFN-query.scw PDB file : Tito_Scwrl_1ZFN.pdb: