Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINCGAKMLIQRGGLKVVAGLGISGV-SAVNFLHEQGYQVAVTDSRPTPPGHDQIPA-GVKTSFGQLDQELLLQAEEIILSPGLAPQLPEIQAAIAKGISVVGDIQLLRRAT-DVPIVAITGSNAKSTVTTLIGLMAKDAGKKV-AVGGNLGRPALD-LLKDQPELLVLELSSFQLETTSHLNAEVAVVLNMSEDHLDRHG-NMLGYHQAKHRIFQGA---KKVVFNRDDALSRPLVPD-TTPMQSFGLNAPDLNQYGVLR-DA--DGTLWLARGLQRLIKSSDLYIQGMHNVANALACLALGEAIGLPMESMLETLKQFKGLEHRCEYVKT--------VHD-VRYYNDSKGTNVGATLAAIDGLGAAIEVKKGKVALILGGQG--KGQD-FSPLRSSIEKYAKVVVLI-----------GEDAPVIEQAIQGA--TKILHAATLKEAVELCQRETQAEDVVLLSPACASFDMFKSYNDRGQQFVACVNSLV 2F00 Chain:B ((10-469)) ---RSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGYQISGSDLAPNPV-TQQLMNLGATIYFN-HRPENVRDASVVVVSSAISADNPEIVAAHEARIPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAGVHARLGHGRYLIAEADESDAS-FLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFLHNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSED--ADVRVEDYQQIGPQGHFTLLRQDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEFPLEPVNGKSGTAMLVDDY-GHHPTEVDATIKAARAGW--PDKNLVMLFQPHRFTRTRDLYDDFANVLT-QVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEMLAPVLTGNDLILVQGAGNIG---------------------

General information: TITO was launched using: RESULT: Template: 2F00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2417 3641 1.51 8.63 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : 1.51 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_2F00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F00-query.scw PDB file : Tito_Scwrl_2F00.pdb: