Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYFELVRSMDVLEKITLPGKPKYLKLAEDF-DFYELFKKIENQFSTCFIFESLGEEDYLSRHHIIGFDPEKFFYSTAVNELCVKDCTTNTILKYSCDHAYYKLREIVPQ-N----IIS-KHYSGGLVGFIGYNAISFFEPTLDVKNN-EDFETFKFGLYLDGLVYDKLTGEIFYFYYKES--RFNEILDILNSDINYNNNIKVQYLGNSVTKEEYENSVYKVKEYIKSGLIFQCEVGFKTDYIITGNHLEIYSKLREVNPSPHMYFMKFDDQKIIGASPELLFRLHNKEMETFPLAGTAKRGKDEIEDRKYARKLLNDPKEIAEHNMLVDLHRNDIGRVAQFGTVKVRKLMDIKRFSHIQHISSEIVGIIKEDEDMFSALASNFPAGTLSGAPKIEAMKIIDEIESSGRGVYGGAIGEFCFNGDCTFAIPIRSIFIKADRAYIQTCGGNVFDSNPEDEYIEIERKLAAMKTVLKNFEV 1K0E Chain:A ((3-452)) ----------------TLSPAVI--TLLWRQDAAEFYFSRLSH-LPWAMLLHSGYADHPYSRFDIVVAEPICTLTTFGKETVVSESEK----RTTTTDDPLQVLQQVLDRADIRPTHNEDLPFQGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDVNARRAWLESQQF--SPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRFHATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGTLPR--------------ANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPFPAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCGSIGYLSFCGNMDTSITIRTLTAINGQIFCSAGGGIVADSQEEAEYQETFDKVNRILKQLE----

General information: TITO was launched using: RESULT: Template: 1K0E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2683 -5517 -2.06 -12.95 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -2.06 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_1K0E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1K0E-query.scw PDB file : Tito_Scwrl_1K0E.pdb: