Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIHPDPQINRLNVLGEPLASCCFDPITGYFRNGFCHTAVTDLGQHTVCAQMTSEFLNFSQKVGNDL-ITPLPEAGFPGLQPGDFWCICVTRWVEAYQAGQAPPVKLHACHQAVLSYVPLDVLMEFAV 1MWP Chain:A ((28-123)) --LLAEPQIA---------MFCGRLNMHMNVQNG---KWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQN--WCK----------RGRKQ------CKTHPHFVIPYRCLVGEFV

General information: TITO was launched using: RESULT: Template: 1MWP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 404 -28546 -70.66 -300.48 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -70.66 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.183

(partial model without unconserved sides chains): PDB file : Tito_1MWP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MWP-query.scw PDB file : Tito_Scwrl_1MWP.pdb: