Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRGVNKVILVGTLGRDPETKTFPNGGSLTQFSIATSEVWTDKNTGERKEQTEWHRIVLHNRLGEIAQQFLRKGSKVYIEGSLRTRQWTDQNGQERYTTEIRGDQMQMLDARQQGEQGFAGGNDFNQPRFNAPQQGGGYQNNNQGGGYGQNNGGYDGQGGFGNGGNSPQGGGFAPKAPQQPASAPADLDDDLPF 3UDG Chain:C ((128-231)) --AMNEVLVLGNVTRDPEIRYTPAGDAVLSLSIAVNENYQD---GQRQEKVHYIDATLWRDLAENMKE-LRKGDPVMIMGRLVNE-----------STRVEATRVEAL-------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3UDG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 378 -43975 -116.34 -483.24 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : -116.34 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.634

(partial model without unconserved sides chains): PDB file : Tito_3UDG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UDG-query.scw PDB file : Tito_Scwrl_3UDG.pdb: