Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIAPRQGLFASLLIISFALHTFLLVIATTHQLNENRASQGQLMTSQLVADSLSELEPANTVSLALIANRYATNPSVASIRILDANKQVLATSGMSKTRQGEIFVRDALQNEKKVGSIEITLIQPSIGEILRTQWLAILASLFLHVLLWLAYRAIARPTRSEYLARINEENRLKHEIQELTQALALEKQNTVTLVAQAQQQAKVKPIVRSQPEKSLESTDQNTLALNIQFYDPKQLLSSV----NQSVSVPYFKLCQLFLNKSIEL---CTKHYHLKATDIDVVDEFHAEGATLAIST--S--HPHAVECLLMVGTVFQLLSDVLYKRYREDKRFALQTRSA-------VCNAVEA----MQI-DAKEAAQRLAQHLHAKESALYLDNEQLKAIQDSYQLVAMPNPSNVMTRHAFMINGMNAECAELAQNIRTEILMGKKSIPQNASPSSAAS 1CUL Chain:B ((6-178)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HQSYDCVCV-MFASIPDFKEFYTESDVNKEGLECLRLLNEIIADFDDLLSKPKFSG----------VEKIKTIGSTYMAATGLSA-ERQYMHIGTMVEFAYALVG----KLDAINKHSFNDFKLRVGINHGPVIAGVIGAQKPQYDIWGNTVNVASRMDSTGVLDKIQVTE--ETSLILQT---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1CUL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 562 -32847 -58.45 -234.62 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.55 3D Compatibility (PKB) : -58.45 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.328

(partial model without unconserved sides chains): PDB file : Tito_1CUL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CUL-query.scw PDB file : Tito_Scwrl_1CUL.pdb: