Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDILENPLELCGFAFIEFVSKE--NELDPIFETIGFSKVAKHKS------KKAYLWRQGNINIILNYQPESY---------------------ASFFFNEHGPSACAMGFKTRDAAKAFKKAVELGAEPMYSKVGP-MELNIPAIKGIGGMPIFLVDCDI-YENDFVFFDDAQRNPEGAGLKEIDHLTHNVYKGRMEYWANFYEKIFNFQEIRYFDIKGEYTGLTSKALTAPDGMIRIPLNEDSDKGNGQIAEFLADFNGEGIQHIAFICDDLISTWDKLKGL----GLKFMTPPPNTYYEMLEERLP--EHGEPTEELKQRCILLDGSTKDGQKKLLLQIFSENMIG--PVFFEFIQRKD--------DDGFGEGNFKALFESIERDQIRRGVLEAK 1SQD Chain:A ((18-410)) ----SDKFKVKRFHHIEFWCGDATNVARRFSWGLGMRFSAKSDLSTGNMVHASYLLTSGDLRFLFTAPYSPSLSAGEIKPTTTASIPSFDHGSCRSFFSSHGLGVRAVAIEVEDAESAFSISVANGAIPSSPPIVLNEAVTIAEVKLYGDVVLRYVSYKAEFLPGFERVEDASSFPLDYGIRRLDHAVGNVP--ELGPALTYVAGFTGFHQFAEFT-----SGLNSAVLASNDEMVLLPINEPVHG-KSQIQTYLEHNEGAGLQHLALMSEDIFRTLREMRKRSSIGGFDFMPSPPPTYYQNLKKRVGDVLSDDQIKECEELGILVDRDD----QGTLLQIFTKPLGDRPTIFIEIIQRVGCMMYQSGGCGGFGKGNFSELFKSI-------------

General information: TITO was launched using: RESULT: Template: 1SQD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1708 42784 25.05 132.87 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 25.05 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.428

(partial model without unconserved sides chains): PDB file : Tito_1SQD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SQD-query.scw PDB file : Tito_Scwrl_1SQD.pdb: