Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMSPSSSGHVNMMKGQPFHVKYREIIRRMSFLIGALLVFRLGAHIPVPGINNAALENLFHANQGTILGLFNMFSGGALERMSILALGIMPYISASIIVQLMST-VIPSLEALKKEGEQGKRKINQYTRQGTLLLAVVQAVGMCAGLIGQGITLSSGLAFYIPAVTSLVAGTMFLMWLGEQITERGIGNGISMIIFAGIVAGLPKLIMQ-------SVSSVDNGQTSLIGLVIFGLLSLGVLAAIVFIEKAQRRIPVNYAQKQQGRRIFTAQQTHLPLKINMAGVIPAIFASSLLLFPASLGQWVGSAD-P--NAG-LIKRSLQDLALVLSP--------GQPLYLVLF----GALIIFFCYFYTA-LVFSPKEVSENLKRSGAYVPGIRPGE-QTARYLDHILNRLTFIGAIYIAVICLMPMILQSSFGIPFYLGGTSLLIVVVVVMDFMAQLQAHLTFHQYDNQTLMRKTTAHPKG 1RH5 Chain:A ((8-423)) ----------LEKIPEVELPVKEITFKEKLKWTGIVLVLYFIMGCIDVYTAGAQI---------PAIFEFWQTITAS--RIGTLITLGIGPIVTAGIIMQLLVGSGIIQM---DLSIPENRALFQGCQKLLSIIMCFVEAVLFVGA----GAFGILTPLLAFLVIIQIAFGSIILIYLDEIVSKYGIGSGIGLFIAAGVSQTIFVGALGPEGYLWKFLNSLIQGVPN-IEYIAPIIGTIIVFLMVVYAECMRVEIPLAHGRIK-------GAVGKYPIKFVYVSNIPVILAAALFANIQLWGLALYRMGIPILGHYEG-GRAVDGIAYYLSTPYGLSSVISDPIHAIVYMIAMIITCVMFGIFWVETTGLDPKSMAKRI------------SEKAIEHRLKRYIPPLTVMSSAFVGFLATIANFIGAL------GGGTGVLLTVSIVYRMYEQLLRERT-------------------

General information: TITO was launched using: RESULT: Template: 1RH5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1663 -239860 -144.23 -634.55 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -144.23 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_1RH5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RH5-query.scw PDB file : Tito_Scwrl_1RH5.pdb: