Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPQYKAPLRDMQFVLHELLNAEEHYAK-----LPDFQGNVSRELVDQYLEAAANFCENELSPLNQVGDREGCTWNDGVVTTPTGFKEAYQKYIELGFPSLSAEEQYGGQGLPNSLGIAISEMVGSSNWAWGMYPGLSHGAVRTL---EHHGSEEQKNTYLPNLVSGV----WTGTMCLTESHAGSDLGI-IRTKAEPNADGSYAISGEKIFISAGEHDMAENIIHIVLARLPGAP----KGTKGISLFIVPKFNVNADGTLGERNAVRCGSIEHKMGIHGNATCVINFDQAKGYLIGPENRGLNCMFTFMNTARIGTAVQGLSASESSFQGALTYAKERLAMRSLSGPKAPEKEADPIIVHPAVRNMLLTQKAFAEGGRALVYLLAQYADIVEKGETEEERKFADNILSLLTPIAKAFLTETGSESAKHGVQVFGGHGFISEHGMEQIVRDTRIACLYEGTTEIQALDLLGRKVLQTQGAMLRDFTKIVHKFVEANKD-NAAMKEFVEPLAALNKEWGDLTMQIGMRAMQNPDEVGAAAVDYLYFSGYVTLAYLWARMALVAQEKLAAGTTDVDFYNAKVTTARFYFKKILPRVRSHVDVIAGGLDPLMALDAEHFAF 4Y9J Chain:A ((25-565)) -HNPYKDDPILDRTLRRLLPES--EYMRVAADLSKFG-----DRITSEVEHLGRQA-ELEQPRLEHQDAWGKRV--DKLIVCNEWHKLKQICAEEGVISIGYEDSVDPFVRRIHQ-VAKLFLFSPSAGLVSCPMAMTDGAVKTLTSLNLYGKHKLATEAVDRLRSRDPSKAWTSGQWMTEKKGGSDVAGGCDTYAVQIDKDTYRLHGYKWFSSA-VD--AD--VALTLARIVDSDGNALEGSRGLSLFLLKIRDES-----GNLNGIQMVRLKNKLGTKQLPTAELLLDGAIAERIGDQGRGVAGISNMLNITRIHNAVASLGYMRRIISLARDYSTKRVVFG------------QTQSKWPLHTTTLAKMEVDTRGSMLLLFEAARLLGLSEAGKS---SDVEAMMLRLITPVLKLYAGKQAVPMVSEGIECFGGQGYMEDTGLPTLLRDAQVTPIWEGTTNVLSLDVL-RVFSGKEN-ILLAFGKRVEQLLGNTKTEDEKLKKSKEAVESALKQLQKLLVKASDSAIQGETRIDSVARHIAFTIARIYSGALLIDHASDSSVAN----------QSDIEVAYRYCCEQ----------------------------

General information: TITO was launched using: RESULT: Template: 4Y9J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2822 47026 16.66 89.91 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 16.66 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_4Y9J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y9J-query.scw PDB file : Tito_Scwrl_4Y9J.pdb: