Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MPIAKLKREKRKDPLWGI-------ILSIIVGSVLMIYPLS---------YDISGWRPLFMLMIMLFWVVCQPTWCGVWFAFGMGIFTDLLLDAPLGLNALSFVI--------VTFITRFLIRERR--ILTFGNLWTIATLVIIAHLAFMWVTQTMGGIHFSIARHWQPLMTSILTWP----VVYYCLAKWRI------------------------------------------- 2REB Chain:? ((3-268)) DENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGLAARMMSQAMRKLAGNLKQS--NTLLIFINQTGGNALKFYASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGI

General information: TITO was launched using: RESULT: Template: 2REB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 545 -73957 -135.70 -459.36 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -135.70 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_2REB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2REB-query.scw PDB file : Tito_Scwrl_2REB.pdb: