Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDLFLNRKSFVVKSLAITVTALMMSGANAATSDKEEIRKLRQEVEALKALVQEQRQVQQQQQQVQQQQQVQLAEVKAQPQPAAAPASPLASLKSKTGADVNLYGFVRGDANYIIEGADNDFADVSKSNGKTQDKLRATAKTTRLGLDFTAPV-SGAKVGGKIELDFASSDNDKSENVRIRHAYLTYN----NWLFGQTTSNFLS-SHAPEMIDF--STN-------I------GGGTTRIPQVRYNYKLAPATQLFVSAEEGNSSATGDSVKYRLPVLTAKVTQGYADGNGNASARAFVENYKSGA---GDDKTGWGIAAGTDFKVSDPLKLFADASYVVGNSNYLYGSNSAYTLVNDNIEQNEFVAVQVGGTYKILPNLRSTLAYGAQFADDGTDYAKNNVTANEKVQQAWINFIYTPVKPIDLGVEYVNGKRDTFEG--------------KSYKDNRVGLMAKYSF 2FGQ Chain:X ((3-332)) ---------------------------------------------------------------------------------------------------SVTLFGIVDTNVAYVNKDAAGD---------SRYGLGTSGASTSRLGLRGTEDLGGGLKAGFWLEGEIFGDDGNASGFNFKRRSTVSLSGNFGEVRLGRDLVPTSQKLTSYDLFSATGIGPFMGFRNWAAGQGADDNGIRANNLISYYTPNFGGFNAGFGYAFDEKQTI-GTADSVGRYIGGYVAYDN--GPLSASLG--LAQQKTAVGGLATDRDEITLG--ASYNFG-VAKLSGLLQQTKFKRDI--------------GGDIKTNSYMLGASAPVGGVGEVKLQYALYDQKAI----------DSKAHQITLGYVHNLSKRTALYGNLAFLKNKDASTLGLQAKGVYAGGVQAGESQTGVQVGIRHAF

General information: TITO was launched using: RESULT: Template: 2FGQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1608 53370 33.19 182.77 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain X : 0.62 3D Compatibility (PKB) : 33.19 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.162

(partial model without unconserved sides chains): PDB file : Tito_2FGQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FGQ-query.scw PDB file : Tito_Scwrl_2FGQ.pdb: