Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MTQYQPPHINRK-LNVQAWWLTALLISINVGLFIWQIISGV-DISDPAIKDALHWGADFTPLTFSGQPERLFTSMFFHFGIIHLLLNMWALYIFGNVAEALFGRLYFIGLYLLAGLFGSLLSSYI--NIQNGHELLQHF------DQSLLPHVSAGASGAVMGLGAALTVLSLFPPLPHQAYILDKKALLMIMAINLIFGFVATGINN-----AAHVGGMIMG-------ALLALIW---YLSYRTPFKSLL---KFLGLVGGL----FMTVGFYVYCNQLNSPLLPLW----HEVLIQNPEILP-------- 3P4G Chain:A ((23-323)) HSFDHYIGSAFDASNNNVAVTGNVSATLNVLAGDDKVSIDGNVEDVLVAANVAVLDMGTGNDQLYVAGDV----------LGKIDAGTGNDEIYIKGDVSAAVDAGTGNDEVYIGGNLSGDLDAGTDNDNIQIGGDVNAALNAGTGNDNLIIGHDVSG----IVNMGTDNDTVEVGRTINASGKVLLDTGDDSLLVSGDLFGEVDGGTGNDTIIIAGKVSGNIQGGTGNDIVRVQSQVWAEANISLGTGDDVLIVEHELHGTVAGNEGDDSIYLKFYTKEQYNNNSDLRNRVANFEHIRVSDGVVKGSPADFADY

General information: TITO was launched using: RESULT: Template: 3P4G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1826 -99449 -54.46 -390.00 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -54.46 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.160

(partial model without unconserved sides chains): PDB file : Tito_3P4G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P4G-query.scw PDB file : Tito_Scwrl_3P4G.pdb: