Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MALY----EIPLLDRN-QKFFIK----LNKVNYQLK-LVYLKRWYLD---------IYQANAEPIARSIPL----------VSGIDILSPYSH-LING-----SMYVQNLNEDESQSFNDLGTNI-KLFWQDPE------------- 1O22 Chain:A ((6-154)) ILEILYYKKGKEFGILEKKMKEIFNETGVSLEPVNSELIGRIFLKISVLEEGEEVPSFAIKALTPKENAVDLPLGDWTDLKNVFVEEIDYLDSYGDMKILSEKNWYKIYVP-YSSVKKKNRNELVEEFMKYFFESKGWNPGEYTFSVQEI

General information: TITO was launched using: RESULT: Template: 1O22.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 157 1089 6.94 11.23 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 6.94 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.142

(partial model without unconserved sides chains): PDB file : Tito_1O22.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O22-query.scw PDB file : Tito_Scwrl_1O22.pdb: