Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTLPLSQYIFPDPEESDPDGHGLICIGADLSPSTLYEAYTHGLFPWFNEDEPICWWNPEPRCIIYPQNYKPSKSLIRNMKKYDYTITVNRAFEQVIRSCSLPRSYANETWISEDIIEGYCGMFDAGYGYSVEVWQEEQLVGGLYGVTIGKGCFGESMFSTQTDVSKMAFYTLM--LIGQENQLPWVDCQLVNSHLISLGACTLSRQEYLKSLQDVIIHPSINWKKYQERVFSSKTIALNAKLME 2Z3L Chain:B ((12-213)) ----------FPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGCASDRE----TWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFSRMENASKTALLVFCEEFIGHGGKL--IDCQVLNDHTASLGACEIPRRDYLNYLNQM-----------------------------

General information: TITO was launched using: RESULT: Template: 2Z3L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 998 -109094 -109.31 -550.98 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -109.31 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_2Z3L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z3L-query.scw PDB file : Tito_Scwrl_2Z3L.pdb: