Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPNQQTIHHFDVIIVGSGGAGLSLALSL-PGHFNIAVLAKAALTEASTFYAQGGVAAVLDET--DSIQQHINDTMIAGAHLCEMDAVQHTVEGGRPSVDFLLKQGVQFTLDEDEQLHLTREGGHSQ--------RRIIHSADATGKAISTTLVERAKERKNITIFENYIAIDLITSHKLGHTEQTNRAIGLYALDESTEKVHTFLAPFTALACGGAMKAYLYTSNPDIATGDGIAMAYRAGCRVANMEFNQFHPTCLYHPQARSFLITEAMRGEGAYLRLPDGERFMLRFDDRA-ELAPRDIVARTIDHEI---KRLGIR--HVWLDITHKSPEFIKEHFPTLY--ARLLELGIDITKDMIPVVPAAHYTCGGVVVDSNSQT----------DIEGLYAIGETSYTGLHGANRMASNSLLECFVYGMSAAKDIESKFDKNFKLPEVPTWDDSQVTNPDEDVVILQNWD----ELR----STMWNYVGIVRTTKRLERALHRIEMLKREITEYYQDYRV---SKNL---IELRNLVLVSEMIVRCAMHRKESRGLHYTLDYPETSSEIRKTVLTPPNFAVEQPLVNTSV 5C3J Chain:E ((40-584)) -------HAYDVVIIGAGGAGLRAAMGLGEAGFKTAVVTKMFPTRSHTTAAQGGINAALGSMNPDDWKWHFYDTVKGSDWLGDQNAMHYLTRNAVEAVTELENFGMPFSRTPEGKIYQRSFGGQSNNYGKGGVAKRTCCVADRTGHSMLHTLYGNSL-RCHCTFFIEYFALDLLM--------DKGRCVGVIALCLEDGTIHRFRSKRTIVATGGYGRAYFSCTTAHMNTGDGTALATRAGIALEDLEFIQFHPTGIY---GVGCLITEGSRGEGGFLVNSEGERFMERYAPKAKDLASRDVVSRAETIEIMEGRGVGPEKDHIYLQLHHLPAEQLHQRLPGISETAKIFA-GVDVTKEPIPVIPTVHYNMGGIPTNYKAQVIKYTKEGGDKIVPGLYACGECACHSVHGANRLGANSLLDAVVFGRACSINIKEELKPDEKIPELPEGAGEE-SIANLDAVRYANGDVPTAELRLTMQKTMQKHAGVFRRGDILAEGVKKMMDLFKELKRLKTTDRSLIWNSDLTESLELQNLMLNATQTIVAAENRKESRGAHARDDFPKREDE----------------------

General information: TITO was launched using: RESULT: Template: 5C3J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 3143 -29295 -9.32 -58.36 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain E : 0.76 3D Compatibility (PKB) : -9.32 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_5C3J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C3J-query.scw PDB file : Tito_Scwrl_5C3J.pdb: