Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEKLQKVLARIGLGSRRYMEEVIAAGRVSVNGRVAQVGERIEPGDELRIDGRKVQFQIEDEIRRRVLIYYKPEGEICSRNDPEKRPTVFDHLPQI-----ANDRWVMVGRLDINSTGLLLFTNDGELANRLMH--PSNEIEREYAVRVMGEVTPQ-LRQ-NMVN---G---VEL-ED---GPAKFESFSEIGGEGINRWYQVVVKEGRNREVRRIFESQGLKVSRLLRTRYGTVILPRELRTGRWMELDKTDIDNLAKSVELKPRQGTGLFGMAKRRTERMTEKPMAARRGGYLRQQRRDDEKEAPANTGNQRKSTGFNRGFKKF 1PRZ Chain:A ((83-303)) -----------------------------------------------------PLDIVYED---EDIIVINKPRDLVVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVESLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEH---FRVHTRLRLRLETGRTHQIRVHMAHITHPLV--GDPVYGGRPRPPKGASEAFI-----STLRKFDRQALHATMLRLYHPISGIEMEWHAPIPQ---------------------------------------

General information: TITO was launched using: RESULT: Template: 1PRZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1005 19828 19.73 98.16 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 19.73 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_1PRZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PRZ-query.scw PDB file : Tito_Scwrl_1PRZ.pdb: