Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTFAPFFQVLGISITLCTQAVFADEGISTQEADSLIKDDIA-STQVLQEICPTFVGANKKLETNTQKIIAMYLSGYSNKSITFSALQNDSEYKTLLSEARQAAKEMDHHEQHELCEEVVNYKD 1TBX Chain:A ((3-96)) ---STPFFY----PEAIVLAYLYDNEGIATYDLYKKVNAEFPMSTATFYDAKKFLIQEGFVKERQERGEKRLYL--------TEKGKLFAISLKTAIETYKQIKKRHHH---------------

General information: TITO was launched using: RESULT: Template: 1TBX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 358 -13044 -36.43 -140.25 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -36.43 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_1TBX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TBX-query.scw PDB file : Tito_Scwrl_1TBX.pdb: