Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MGLNMPEPTSTTAATTTSLAAVSL--------LPFINGNALLGAVLGAAFVAYLE-----KDLSAKQRIVFMLLCVGFG---YLLGPEITSRTQYI--SSDATASMVAAIFAIYILIKLLDWVKNSTLAQLWKTFRGGGNS-- 4FFE Chain:X ((0-149)) GHKLAFNFNLEINGSDTHSTVDVYLDDSQIITFDGKDIRPTIPFMIGDEIFLPFYKNVFSEFFSLFRRVPTSTPYEDLTYFYECDYTDNKSTFDQFYLYNGEEYTVKTQEATNKNMWLTTSEFRLKKWFD--GEDCIMHLRSLVRKMEDSKR

General information: TITO was launched using: RESULT: Template: 4FFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 461 9805 21.27 81.03 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain X : 0.67 3D Compatibility (PKB) : 21.27 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.056

(partial model without unconserved sides chains): PDB file : Tito_4FFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FFE-query.scw PDB file : Tito_Scwrl_4FFE.pdb: