Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MKMFTKLALVSSLAISANAMAMQSMDDAALSA----ATGQDG-INIGIA-LGTSGISIDKLYLHDNDGLATTTGITGATGTAGALAISDVTLKQTGTGNLLDLAIDT-NGASS-TNGAFLNVAATVGAVDIHVGSIGVGTSGTVNE-TTALRGITETA-PTEIISGLDLSLG-QITANVQLGSTPQ---GAMIKVDSALKGG----LTISNLGINDAAGGGSILLDKVMVR----GAGNATGDLD-VKADIS--VTGNGLQVKSTSAQDMNVYVGGVHLGTNTKASDGTWGTGAVKAASIGDLEIQ-GLNVANTTITISGH 4OVY Chain:A ((1-305)) EEPLPSWNDSAARRAILEYVKSVTTEGSPRFVPVSERIVTFDNDGTLWCEQPMYVQLAFALDRVRLLADKHPEWRTE----EPFRAVIEKDLPALAKLGAKGLTEL---TMATHAGMTDDEFENIVTEWIRKARHPKFHRPYTECVYQPMLELLAFLRQHEFKTFIVSGAGIEF-MRPWAKEVYGIPPEQVIGSSVKLKYELRDGKPVLVRLAELNFIDDQAGKPVGIRQVIGRRPVMAVGNSDGDYEMLEYVTSGPANGLGLIVHHTDAVREFAY--------DRQSPFGRLDRALTDATSKGWIVIDMQRDWK--VIFPES-

General information: TITO was launched using: RESULT: Template: 4OVY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1361 36971 27.16 133.95 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 27.16 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.138

(partial model without unconserved sides chains): PDB file : Tito_4OVY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OVY-query.scw PDB file : Tito_Scwrl_4OVY.pdb: