Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MDHVSRVNKVVDKIVPLSVSFLFVILLFFLCVLFVIIKEKWGNC 2P1L Chain:B ((1-24)) GSGTMENLSRRLKVTGDLFDIMSG------------------------

General information: TITO was launched using: RESULT: Template: 2P1L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1 -164 -163.50 -8.18 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.48 3D Compatibility (PKB) : -163.50 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.48 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_2P1L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P1L-query.scw PDB file : Tito_Scwrl_2P1L.pdb: