Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGIVSNDRGK-LKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFKFHDQSIGLAHNGNLINARSLRKEL-EENGAIFHSNSDTEILMHLIRRSE---------ETDFLNKLKSALNE-VKGGFAYLLMT-ETAMIAALDPNGFRPLAIGQM----KNGAYVIASETCALEVIGARFIRDVQPGEIVIINDEGIQIDCFTKDTQLS-ICSMEYIYFARPDSNIAGINVHTARKNMGRNLAKE-----SPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELREQGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKYPCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCMAYFNGDYPTPLYDYEEKYQESLKEKVSFF 1ECB Chain:C ((1-478)) -----------CGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFRLRKANGLVSDVF-EARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVN-SPYGITLAHNGNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFA-SELDNFRHYPLEADNIFAAIAATNRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLGFDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSARVNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRTFIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYLASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAENPDIQQFEC-SVFNGVYVTK--DVDQGYLDFL-------

General information: TITO was launched using: RESULT: Template: 1ECB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2759 8068 2.92 17.73 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : 2.92 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_1ECB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ECB-query.scw PDB file : Tito_Scwrl_1ECB.pdb: