Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------TVASR-RDDCWVSLFQYKANGEREAIYVDYSP-PNSLVIFVHDGWRLNVQLDQHCRSLFPNDIRKQ----LVEG--FG------VHSYSKMNEVAGQKPLARSQ--- 1NOA Chain:? ((1-113)) AAPTATVTPSSGLSDGTVVKVAGAGLQAGTAYDVGQCAWVDTGVLACNPADF-SSVTADANGSASTSLTVRRSFEGFLFDGTRWGTVDCTTAACQVGLSDAAGNGPEGVAISFN

General information: TITO was launched using: RESULT: Template: 1NOA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 333 9902 29.73 111.25 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 29.73 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.300

(partial model without unconserved sides chains): PDB file : Tito_1NOA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NOA-query.scw PDB file : Tito_Scwrl_1NOA.pdb: