Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNSKYITCLKRSEGQLRGIQKMIEGDRDCADIVTQLTAVRSSVERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK 5FMN Chain:A ((25-106)) -SLQKLVNRLSRIEGHIRGVKTMVQENRPCPEVLIQVAAVRGALDRVARLILDDHMNECITRAAAEG-NIEQELAELKEALDRF-

General information: TITO was launched using: RESULT: Template: 5FMN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 128 -14568 -113.81 -177.65 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -113.81 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_5FMN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FMN-query.scw PDB file : Tito_Scwrl_5FMN.pdb: