Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------------------------------------------------------MKKISHLCMLLLLLCTTF------FVFNVNYTREVVRIQEMGKTV-----DSLDLY-----LKDINEPAASVLRFFEDVSKEYKVSII---KTDSGDEVVKSGVFDKDTFPYQEFGISSLDFTTDGEGVYSNK--EISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEMDKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQPMMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKP---KDLFPMLWLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSF---KFGLIFNYVMKIGTTIL--LTALLIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFYQDLVEKTQAGYVQSSS-LPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTGIKKVILMPA------STKGEEDKNQLLGKLIAFHSMK--------------YEEQQKRTIEEMDVEIAYYEGDWSFFPYSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKLSDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLL-VTTFLLKRRDIITKKFMGWKL----------------VDRYRPLLVLLLLGYSFPL--------------LVLIFFAHAFLPLLLFAGFTCLDILFV---------LGLASRMEKRSLVELLKGGIL----------------------------------------------------------------------------------------------------------------- 4MT1 Chain:A ((2-1040)) AKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTITLHAIYPGASAQVMEGSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENLAQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNVVPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNIQISAGSIGSLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNIYLKDVAKVGLGMEDYSSSTRLNGVNTTGMAVMLSNSGNAMATAKAVKERLAVLEKYFPQGMSWKTPYDTSKFVEISIEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSI---NVLTMFAMILVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISVFVPLAMFSGAAGNIYKQFALTMASSIAFSAFLALTLTPALCATGFFGWFNKKFDSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVG----VFLFMRLPTSFLPTEDQGFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFAIFKDWNERTASGSDAVAVAGKLTGMMMGTLKDGFGIAVVPPPILELGSGLSINLQD---RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISFADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPLSTIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMEAVQKMVDELGGGYSFEWGGQSSEEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVTGRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAALEAARLRFRPIIMTSFAFILGVVPLYIAAGASSASQRAIGTTVFWGMLVGTLLSVFLVPLFYVVVRKFF

General information: TITO was launched using: RESULT: Template: 4MT1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3047 -78425 -25.74 -113.33 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -25.74 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.188

(partial model without unconserved sides chains): PDB file : Tito_4MT1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MT1-query.scw PDB file : Tito_Scwrl_4MT1.pdb: