Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFVNFFHEKEKIMRYDFGKVYKEIRESKGLTQEEVCGGVL-SRTSLSKIESGKTTPKYENMEFLLRQINMSFEEFEYICQ-LYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQDYLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLKILNTILFSFPIEYLHLITGK-ILQRLEVYKNYQHLYDLRMTILLNLST-LYLYNQDKNMCKQICYTLLEDAKNKKSYDRLAICYVR------IGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQAKER------------ 2AWI Chain:A ((2-301)) --------------FKIGSVLKQIRQELNYHQIDLYSGI-MSKSVYIKVEADSRPISVEELSKFSERLGVNFFEILNRAGMNSVNETGKEKLLISKIFTN-------PDLFDKNFQRIEPKRLTSLQYFSIYLGYISIAHHYNIEV-PTFNKTITSDLKHLYDKRTTFFGIDCEIVSNLLNVLPYEEVSSIIKPMYPIVDSFGKDYDLTIQTVLKNALTISIMNRNLKEAQYYINQFEHLKTIKNISINGYYDLEINYLKQIYQFLTDKNIDSYLNAVNIINIFKIIGKEDIHRSLVEELTKISAKEKFTPPKEVTMYYEN

General information: TITO was launched using: RESULT: Template: 2AWI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1058 3886 3.67 14.08 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 3.67 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_2AWI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AWI-query.scw PDB file : Tito_Scwrl_2AWI.pdb: