Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAALAAAGGGGLQLVLASY---------GLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGADWIFTNIID------GWDGRRDNQLRIG 1MFI Chain:A ((1-114)) -----PMFIVNTN-------------VPRASVPEGFLSELTQQLAQATGKPAQYIAVHVVPDQLMTFSGTNDPCALCSLHSIGKIG-GAQNRNYSKLLCGLLSDRLHISPDRVYINYYDMNAANVGWNGSTFA-----

General information: TITO was launched using: RESULT: Template: 1MFI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 421 -29621 -70.36 -299.20 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -70.36 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.167

(partial model without unconserved sides chains): PDB file : Tito_1MFI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MFI-query.scw PDB file : Tito_Scwrl_1MFI.pdb: