Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGNRANIEEIL-GNRVELVVGDIAD-AELVDKLAA-QADAIVHYAAESHNDNSLNDPSPFIHTNFIGTYTLLEAARKY----------DIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSPYSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLYGEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAA-----------DAYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKEAVEANYAKTQEIITV 1BXK Chain:B ((2-339)) ----RKILITGGAGFIGSALVRYIINETSDA-VVVVDKLTYAGNLMSLAPVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNIVGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDF----------FTETTPYAPSSPYSASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYGNGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPNKPHGVAHYRDLITFVA------LRYAIDASKIARELGCVPQET-FESGMRKTVQWYLANESWWK-------------------

General information: TITO was launched using: RESULT: Template: 1BXK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1660 -43921 -26.46 -142.60 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -26.46 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_1BXK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BXK-query.scw PDB file : Tito_Scwrl_1BXK.pdb: