Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKVGVGQAHSKIILIGEHAVV------YGYPAISLPLLEVEVTCKVVPAESPWRLYEEDTLSM--------AV----Y-A----SLEYLN-----ITEACIRCEIDSAIPEKRGMGSSAAISIAAIRAVFDYYQADLPHDVLEILVNRAEMIAHMNPSG-LDAKTCLSDQPIRFIK--NV--GFTELEM------DLSAYLVIADTGVYGHTREAIQVVQNKGKD----ALPFLHALGELTQQAEIAISQKDAEGLGQILSQAHLHLK--EIGVSSLEADSLVETALSHGALGAKMSGGGLGGCIIALVTNLTHAQELAERLEEKGAVQTWIESL 4USK Chain:A ((2-326)) -NPTIIRARAPLRLGLAGGGTDVAPYADTFGGYVLNATIDRYA-YAVIKTLTIPAVRFVSTDQQVEKHQLISEPLELNGTLNLHKAVYNHMIRNYNHGKPIALELSTFCDAPAGSGLGSSSTLVVVMIKAFVELLNLPLDDYAIAQLAYRIERVDCGLAGGRQDQYSATFGGFNFMEFYAAARTIVNPLRIKNWVLCELEASLVLFYTGVSRESAKIIQDQSDNVVSHKTAAIEAMHGIKREALVMKEALLKGDFKAFVASMRLGWDNKKNSARTVSNAHIDEIYDAAIRAGAQAGKVSGAGGGGFMLFFVPTEKR-MDLIRTLGEYD---------

General information: TITO was launched using: RESULT: Template: 4USK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1446 -35581 -24.61 -127.07 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -24.61 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_4USK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4USK-query.scw PDB file : Tito_Scwrl_4USK.pdb: