Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEVDTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA 3KFU Chain:G ((5-90)) -ELSPELLRKLETLAKIRLSPEEEALLLQDLKRILDFVDALPRVEEGGAEEA------LGRLREDEPRPSLPQAEALALAPEAEDGFFRVPPV-------

General information: TITO was launched using: RESULT: Template: 3KFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 96 -11101 -115.64 -129.08 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain G : 0.76 3D Compatibility (PKB) : -115.64 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.676

(partial model without unconserved sides chains): PDB file : Tito_3KFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KFU-query.scw PDB file : Tito_Scwrl_3KFU.pdb: