Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHISESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEACKAVVRANEEYVPLYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGGLVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKIEEVGSANFF---GITADNEFVTP-LSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDRFVEAG------ACGTAAVISPIGGIQHG-DDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEGWIVKVD 5U3F Chain:B ((50-367)) -----------------------------WHNARVIPYGPIELDPSAIVLHYAQEVFEGLKAYRWADGSIVSFRADANAARLRSSARRLAIPELPDAVFIESLRQLIAVDKAWVPGAGGEEALYLRPFIFATEPGLGVRPATQYRYLLIASPA-------IAPVSVWVSTEYVRACPGGTGAAKFGGNYAASLLAQAEAAENGCDQVVWLDAVERRYIEEMGGMNIFFVLGSGGSARLVTPELSGSLLPGITRDSLLQLAID-AGFAVEERRIDIDEWQKKAAAGEITEVFACGTAAVITPVARVRHGASEFRI--ADGQPGEVTMALRDTLTGIQRGTFADTHGWMARL-

General information: TITO was launched using: RESULT: Template: 5U3F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1570 -76356 -48.63 -254.52 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -48.63 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_5U3F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5U3F-query.scw PDB file : Tito_Scwrl_5U3F.pdb: