Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSRDIKNMNLSKVREEDSA---YYVLNNGS----ISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGLAQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKC---FEELKKFAPPFFFEE 3LAJ Chain:F ((20-170)) -----GRQARIVAILSSAQVRSQNELAALLAAEGIEVTQATLSRDLEELGAVKLRGADGGTGIYVVPEDGSPVRGVSGGTDRMARLLGELLVSTDDSGNLAVLRTPPGAAHYLASAIDRAALPQVVGTIAGDDTILVVAREPTTGAQLAGMFENLR----------

General information: TITO was launched using: RESULT: Template: 3LAJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 495 -29899 -60.40 -212.05 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain F : 0.78 3D Compatibility (PKB) : -60.40 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_3LAJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LAJ-query.scw PDB file : Tito_Scwrl_3LAJ.pdb: