Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIG--VKKSH-DLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWGAYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANTFLKETGQEPIDWLR 4SKN Chain:E ((7-220)) ----SWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVILGQNPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGDLSGWAKQGVLLLNAVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPRSVYRGFFGCRHFSKTNELLQKSGKKPIDW--

General information: TITO was launched using: RESULT: Template: 4SKN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1052 -43116 -40.98 -204.34 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain E : 0.87 3D Compatibility (PKB) : -40.98 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_4SKN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4SKN-query.scw PDB file : Tito_Scwrl_4SKN.pdb: