Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKNIYFVVLDLHTTDRDKIIQLFKDWTDYSAKLVEGELVKKDGQNALFPPSDTGETVG-LNPHRLTLTFGVSAS-FLKRMNLENKRPRLFRDLPLFPKEQLREKYTGGDIVIHACADDEQIAFHAIRNLIRKGRNAVPLRWSQSGF----AAIGDRMETPWNLFGFKDGTANPTKEQD--FDRVIW--ADSKD--WMENGSYMAVRRIQMFLETWDRTSLEEQENTFGRYKESGAPFGKKNEFDEVDLSLLPD------DSHVCL---AKEVDKPLLRRSYSYSDGIDEKTGQFDTGLLFISFQKDPDN-FVKVQTNLGATDKMNEYITHIGSGLFTCFGGVEKGG-YIGQKLLEG 2Y4D Chain:B ((49-401)) -QAAMMLVAFDVLASDKADLERLFRLLTQRFAFLTQGG-AAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHAARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANP--SESSLMLRRGYSYSLGV-TNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNG-EALEEYVKPIGGGYFFALPGVKDANDYFGSALL--

General information: TITO was launched using: RESULT: Template: 2Y4D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1575 39805 25.27 122.10 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : 25.27 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_2Y4D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y4D-query.scw PDB file : Tito_Scwrl_2Y4D.pdb: