Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVEIKNLSLDYG--EEHILDDISLSIAEGECVLFTGKSGNGKSSLINSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLELLFYLENIGLARE-EMDRRLKDMLKIFPIKNLL------------NRNIFNLSGGEKQILCIAASYIAGTKIIVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLKKTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFIFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL 2ONJ Chain:B ((340-563)) -IDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRF---YD--VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVK---------ENILLGRPTATDEEVVEAAKMANAHDFIMNLPQGYDTEVGERGV-KLSGGQKQRLSIARIFLNNPPILILDEATSALDLESESIIQEALDVLSKDRTTLIVA-HRLSTITH-ADKIVVIENGHIVETGTHRELI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ONJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1049 -28380 -27.05 -135.79 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -27.05 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_2ONJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ONJ-query.scw PDB file : Tito_Scwrl_2ONJ.pdb: