Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVMSLITHRRFISCNENIKHYKRLIDKAEKCVNDLMAELNSVITTVTGIGNRLGAVILAEIQNIHAF--------DNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLR 3U4E Chain:G ((1-122)) MTTFKLAACVTLRC-TNATINGSLTEEVKNCSFNITTELRDKKQKAYALFYRPSEYILINCQTTTTEAVDAATAAKVFKQYANDNGIDGEWTYDDATK--TFTVTEGLEVL-

General information: TITO was launched using: RESULT: Template: 3U4E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 343 -16518 -48.16 -165.18 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain G : 0.63 3D Compatibility (PKB) : -48.16 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.155

(partial model without unconserved sides chains): PDB file : Tito_3U4E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U4E-query.scw PDB file : Tito_Scwrl_3U4E.pdb: