Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTFVDAARSFRTQFDLNFSEKIIVDFFAGGGGASTGLEMGLNRPVYVAVNHNPKAISMHEANHPHAKHYIQDVFAVDPIDICDGHQVGWFHASPDCTHHSQAAGGQPRKKEIRDLSWVVLKFAGKVKPDVISLENVKQILGWGPLIAKRDKATGRVITLDKININGKKVNRIAEPGERVPRHNQFLVPNPKKKGKTWKHFVRSLEQLGYEVEWQKNIIAADFGAPTKRERLFLIARCDGQPI---VWPEKYFSKKPKGNLKKWRSTVECVDFSDLGNSIFDRPQGPLADATLKRIAKGIQKYVIETKEPFFVNSSTPFIGRDFRTSFGHDIREPLATTTAGYGGHSSLISPILVPFITEFANASQQRNWSIDEPLSTICAQVKGGHHGLVTAKLSKDNYKGALRVAAFLINYYGNGDARSITEPMDTITTKDRLVLVTVWIKGEPWAIVDICIRMLKPRELFRAQGVPDSYVIEYGSDGKPLSKKDQVFMVGNSVSPYPMAAIARANNPFITQQIKGAA 2I9K Chain:A ((8-324)) -----------------QLTGLRFIDLFAGLGGFRLALESC-GAECVYSNEWDKYAQEVYEMNFGEK--PEGDITQVNEKTI---PDHDILCAGFPCQAFSISGKQKGFEDSRGTLFFDIARIVREKKPKVVFMENVKNAASHD-------------------------------------------------NGNTLEVVKNTMNELDYSFHAKV-LNALDYGIPQKRERIYMICFRNDLNIQNFQFPKPFEL---------NTFVKDLLLPDSE---V-E----HL---VIDRK--DL---VM------------T-N------------QEIEQT------------TPK--------------T-----VRLGIV--GK----------------------------GGQ-GERIYSTRGIAITLSAYGGGI----FAK-TGGYLVNGKTRKLHPRECARVMGYPDSYKVH------PS-TSQAYKQFGNSVVINVLQYIAYNIGSSLNF------

General information: TITO was launched using: RESULT: Template: 2I9K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1868 -88671 -47.47 -282.39 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -47.47 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_2I9K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I9K-query.scw PDB file : Tito_Scwrl_2I9K.pdb: