Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTPEDISQNKPLLWLMAAACGLCAGVNYYCQPLIHSIQ------QDFAVTQAQAALTVTFAQVSYALGLLFIVPMGDIV-NKAKAIPFLMMLCAVGLFTSAFAV--NLPMLWIGTILAGLFS-VAAQVLIPLATMTVKP--EKTGEIIGFLMSGLLVGILLSTSLAGLFSNLFHWKVVYAISGILMLLLAYTLKS-RLPYVMRM-K-L-NYG---QI-FVS--M-------------------------------------------------AQLLKEEKRLLLRAL----TGAFAFASVSILYSTIALLMTSA-----HHLPDLFIGLV-PLVGIFGALSTRYIGKYADQGYTR----LLTWMGCGLFLLSWICFYFGQT----------LLSAYVIGFALIQLALALVHTSNQSIIFR-LRPDAKSRINAIYMTTYFIGGAAGSALGIFAWNRG--EWTMTCLAGVGLVVFCILFSMIDAFLQKKQMA 4W6V Chain:A ((20-490)) -------------LSGLFLSEMWERFSFYGIRPLLILFMAATVFDGGMGLPREQASAIVGIFAGSMYLAALPGGLLADNWLGQQRAVWYGSILIALGHLSIALSAFFGNDLFFIGLVFIVLGTGLFKTCISVMVGTLYKPGDARRDGGFSLFYMGINMGSFIAPLLSGWLLRTHGWHWGFGIGGIGMLVALLIFRGFAIPAMKRYDAEVGLDSSWNKPT---GRWVTAIMAVVVVIIALISQGVIPINPVMIASLLVYVIAASVTLYFIYLFAFAKMSRKDRARLLVCFILLVSAAFFWSAFEQKPTSFNLFANDYTDRMVMGFE-IPTVWFQSINALFIILLAPVFSWA-------PSSITKFVIGILCAAAGFAVMMYAAQHVLSSGGAGVSPLWLVMSILLLTLGELCLSPIGLATMTLLAPDRMRGQVMGLWFCASSLGNLAAGLIGGHVKADQLDMLPTLFARCSIALVICAAV-------------

General information: TITO was launched using: RESULT: Template: 4W6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1381 -185333 -134.20 -519.14 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -134.20 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_4W6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4W6V-query.scw PDB file : Tito_Scwrl_4W6V.pdb: