Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLAQRLAAVVLTLGLSAGLVGCGFHLKGTNPTATPLVYKKLSL-ELPAKTDDLETQLKVYLTANGVQLSNDN--DA-YVLRVLEYTPRRQLL----NGKLTEVLLRLTVTFQIEDRQGNKITEPRTLTAARSYQYDLATVNTENQQESYLQRIVIDDLAQQITRQISANRLPKAQP 5IXM Chain:F ((8-152)) -------------------------NLRGTT-QVP-TELQKLLLESSDPY-GPLARSIRQQLRLNNVTIVDDAMRKDIPTLRIIGSSESQETVSIFRNGVAAENQLVLHVQAQVLIP-GHDIY-PLQVNVFRTFFDNPLTALAKEAEAEVLRQEMREQAAQQLVRQLLTVHAAEV--

General information: TITO was launched using: RESULT: Template: 5IXM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 498 9906 19.89 72.30 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain F : 0.64 3D Compatibility (PKB) : 19.89 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_5IXM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IXM-query.scw PDB file : Tito_Scwrl_5IXM.pdb: