Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MTHTAVANVKLNWTYFCLILASTFLQ-GSSFVATKILLSSMSPLWVATIRFFIA-ALSLLPLIIYRYFKNPISLFNIPWLKLFVIGLFQTAGVMAF-LNIGLGYTSPSTAAILMASNPLLVVILAMLILGERISIRALVGLIVAFIGVVICIGLGNTNSGGIGRGEVLV-ILASSCWAIATI-INKKFNLHLDPWVITFWQMLLGSLVLFLVALFSQQPFTLPTTESMWLTFLWLAIPASTGAMGLWFAALKIGGAIHTSGFLFLCP-LFSAIITYFVLGTVLTSQELIGGFLIGTGIYVLSRYR- 3I3N Chain:A ((1-274)) YFQSMEAEDFECSSHCSELSWRQNEQRRQGLFCDITLCFGREFRAHRSVLAAATEYFTPLLSGRVEMR---------------------KWSSEPGPEPDTVEAVIEYMYTGRIRVSTGSVHEVLELADRF--------------LLIRLKEFCGEFLKKKL------HLSNCVAIHSLAHMYTLSQLALKAADMIRRNFHKVIQD------EEFYTLPFHLIRDWLSDLEITVDSEEVLFETVLKWVQRNAEERERYFEELFK---------LLRLSQMKPTYLTRHVKPERLVANNEVCVKLVADAVERHALRAEN

General information: TITO was launched using: RESULT: Template: 3I3N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 880 -84751 -96.31 -348.77 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -96.31 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.250

(partial model without unconserved sides chains): PDB file : Tito_3I3N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3I3N-query.scw PDB file : Tito_Scwrl_3I3N.pdb: