Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYCQFFSVLALGLSAASCAV--------TSGLQTY----DIPSEGVYKTDLGTTVNVVK-ISQETLPAIQPAQIDYQRDYASLFKNQQTIYRLSP---GDVLSIQLWAYPEIAPPVNNISNEQSVQAYGYPIDQTGYIQFPLVGRYKAAGKTLAQVNREL-HSQLARFLK---NPDVVVRVVSYEGQRFSVQGSVTKGGQFYLSDQPVSIYTALGMAGGVTT-TGDNTYIQLIRNGRTYNLNTIDLEKAGYSLHKLLVQP--NDTIY--VSTRENQKIYVMGESGKNQALPMRDQGMSLTDALGESLGINPLSGSASRIYVVRT--------NPN---------DRTTEIYHLNLMSIGDFGLANQFRLRSN-----DVVYVDATGL--TRWQRIVNQII--PFSNALYNIDRLGQ------------ 5HTX Chain:A ((14-437)) ---EKLYLGMDFGTSGGRFTVIDEQGEIKAQGKREYPPFMKEESMGWASSWKATLFSLLEDIPVTVRSLVSSISLDGTSATTLILNSESGEVLCQPYLYNQSCPDALPEVKSIAPANHTVCSGTSTLCKLVSWWNTEVPNRESAVLLHQADWLLWLLHGRLGVSDYNNALKVGYDPESESYPSWLLGQPYSQLLPKVQAPGTSIGNLKESFTRQFGFPDDCIVCTGTTDSIAAFLAARATEPGKAVTSLGSTLAIKLLSTKRVDDARYGVYSHRLDDKWLVGGAS--NTGGAILRQLFSDEQLERLSQEINPMVGSPLDYYPLQSSGERFPIADPNLAPRLLPRPESDVEFLHGILESIARIEGKGYKLLKELGATEAEEVLTAGGGAKNDKWIKIRQRVLGLPVKKAVHTEASYGASLLALKGAKQNS

General information: TITO was launched using: RESULT: Template: 5HTX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1919 50554 26.34 140.04 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 26.34 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.132

(partial model without unconserved sides chains): PDB file : Tito_5HTX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HTX-query.scw PDB file : Tito_Scwrl_5HTX.pdb: