Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLPILSFPDPRLRTIAKPVEEVTDEIRQLAADMFETMYAAPGIGLAASQVDRHIQLIVMDLSESKDEPMVFINPKVTPLTEETQPYEEGCLSVPQIYDKVDRPSRVKIEAINLEGQAFEIEADGLLAVCIQHEMDHLNGKLFVDYLSPLKRQRVREKVEKIVRQREREKVAVKR 4AL2 Chain:C ((1-162)) -SVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGET-GIEEGCLSIPEQRALVPRAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVEKLD------------

General information: TITO was launched using: RESULT: Template: 4AL2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 774 -98801 -127.65 -609.88 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain C : 0.88 3D Compatibility (PKB) : -127.65 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.655

(partial model without unconserved sides chains): PDB file : Tito_4AL2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4AL2-query.scw PDB file : Tito_Scwrl_4AL2.pdb: