Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQQSNMQNKFLIDAEIGDDDVTLPNLPAIDVPIVESPVKQEVKAEEVVEAAPSTEPEQPKSGFFGRMKEGLSKTRRNFTDGMVNILIGGKEIDDELLEEVEGQLLVADIGVDATKTIITNLTERTARGDLIYSHSLYKALQEELVALLAP-RVKPLHIDPNKSPYV------ILMVGVNGVGKTTTIGKLAKRLQGEGKKVMLAAGDTFRAAATEQLQIWGERNDIAVVAQGHGADSASVIFDASESARAKGIDVLIADTAGRLHNKSNLMEELKKVKRVMQKIDATAPHEIMLVVDAGTGQNAINQVQEFDQAVGLTGITITKLDGTAKGGVLFNIASRTHVPIRFIGVGEKIDDLRPFSAKSFVAALFETEK 2Q9B Chain:B ((2-303)) --------------------------------------------------------------GFFDRLKAGLAKTRERL---LKAIPWGGNLEEVLEELEM--ALLAADVGLSATEEI---LQEVRASG-------LKEAVKEKLVGMLEPD----------KPKPVEPKGRVVLVVGVNGVGKTTTIAKLGRYYQNLGKKVMFCAGDTFRAAGGTQLSEWGKRLSIPVIQGPEGTDPAALAYDAVQAMKARGYDLLFVDTAGRLHTKHNLMEELKKVKRAIAKADPEEPKEVWLVLDAVTGQNGLEQAKKFHEAVGLTGVIVTKLDGTAKGGVLIPIVRTLKVPIKFVGVGEGPDDLQPFDPEAFVEALLE---

General information: TITO was launched using: RESULT: Template: 2Q9B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1441 24060 16.70 86.55 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : 16.70 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_2Q9B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q9B-query.scw PDB file : Tito_Scwrl_2Q9B.pdb: