Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNPSISLIREIDALLPQTQCGLCGHRDGCLPYAKSIAEGEEANKCVPGGQPVADALAQLLNRPLLPAETSVWPVQADGRPQRMKAIIREDECIGCTKCINAC---PVDAIIGSGKLMHTILTDLCTGCELCIPPCP-VDCIDLVEDTQALPSEQQRFAEQNDLRQRYYAHIQREEKRRIHRKGPVVRAEIDTQLFAQFSQALDDLPSIQLIEKPKEVIVSDAKTTIELAKIRTQIKKLEKQLSVREDAKKRLQLDELQQQLIQLQEV 1H7W Chain:B ((942-1015)) --------------------------------------------------------------------------------EQVVAVIDEEMCINCGKCYMTCNDSGYQAIQFDPETHLPTVTDTCTGCTLCLSVCPIIDCIRMVSRTT--PYEPKR---------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1H7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 278 -27300 -98.20 -389.99 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -98.20 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_1H7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H7W-query.scw PDB file : Tito_Scwrl_1H7W.pdb: