Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------MDLQGCLIVIKTDVIFDESNIIIGLFTIKDDHGIIQ---ISTNGNQVNWDEWVDEAGG----HSLEKAIIYCLNRKPPEDATHYKE------TKKQNSYRYYKKVEEDWFIYVDWRYPLGWQPTGMFDDITKIKPLPQFG----------------- 3ZXU Chain:B ((69-269)) SVQLDDKLLLKLLRRNDNAVSDSSNPLPRVLPSLNIEQRKKYLDITLNDVTVTCEKDMILLRKGSFTASFRIAVENESIRSMAIDLNAFEVELQPIIQYAEDTQNVNVAMMAVVQFLRIKELHEQMISKIVEASKFIRASNNTITLNDLEVSFHCY--WNLPSPYPETLILTN--KVQKILDFLIYQYGIQLGVIKYGSTI

General information: TITO was launched using: RESULT: Template: 3ZXU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 303 4578 15.11 37.22 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : 15.11 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.195

(partial model without unconserved sides chains): PDB file : Tito_3ZXU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZXU-query.scw PDB file : Tito_Scwrl_3ZXU.pdb: