Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MTKKNNAPELHKYRGLTSTEQMVIHQMLISYVREENCRFNIIMTGTAEPYNLVKLTSINFENEASAIWVHFETITGEQIALPIDF-LSRIEFSGQQEI 1V31 Chain:A ((1-93)) GSSGSSGVPEKFKLSTALMDVLGIEVETRPRIIAAIWHYVKARKLQ------------NPNDPSFFNCDAALQKVF-GEEKLKFTMVSQKISHHLSPPPPSGPSSG

General information: TITO was launched using: RESULT: Template: 1V31.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 322 13621 42.30 162.15 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 42.30 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.336

(partial model without unconserved sides chains): PDB file : Tito_1V31.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V31-query.scw PDB file : Tito_Scwrl_1V31.pdb: