Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MFKPISLYI--------GLRYTRARRSNHFISFIA-LVSMVGLTLGV-AVLITVLSVMNGFDREL-K-NRVLGMVPQATVSSTQILTDW---PELVKRVENHPHMTGVAPFTQL-QGMLTAQGQVAGIMVTGIDPKYEKNVSIIQNHIVAGSLDSLKKGEFGIVLGK----DMAD-SLGLRLNDSV-T-LVLPEATPS---PAGVVPR--------FKRFKVVGIFSVGAEVDSMVGYIALYDASTLLRLPDGAQGVRLKLDDIFAAPQVADDIVKNLP-SNFYATNWTYTHGNLFNAIQMEKTLVGLLLVLIIVVAAFNIVSSLVMVVTDKKSDIAILRTLGASPSMITKIFMVQGTVIGV-IGTVA-GTVLGVILALTISDIISWFNNVLGLNLFDAYFVHYLPSYLRWKDVTIIV-IVSLLLSFLATIY---PALRAAKVQPAEALRYE-- 4QT9 Chain:A ((19-432)) LSDDALMDTVQRRTFLYFWEGAEPNSGLAPERYHVDGVYPQNDANVVTSGGSGFGIMAILAGIDRGYVTREEGLARMERIVSFLEKADRFHGAYPHWWYGDTGKVKPFGQKDNGGDLVETAFLMQGLLAVHQYYANG-----NDKEKAIAQRIDRLWREVDWDWYRKGGQNVLYWHWSPTYGWEMDFPIHGYNECMIMYILAAASPTHGVPAAVYHDGWAQNGAIVSPHKVEGIELHLRYQGTEAGPLFWAQYSFLGLDPVG-------LKDEYCPSYFHE-------MRNLTLVNRAYCIRNPKHYKGFGPDCWGL-------TASYSVDGYAAHS-PNEQDDKGVISPTAALSSIV----YTPEYSLQVMRHLYNMGD--KVFGPFGFYDAFSETDN------------WYPQRYLAIDQGPIAVMIENYRTGLLWKLFMSHPDVQAGLTK----LGFNTN

General information: TITO was launched using: RESULT: Template: 4QT9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1966 -39478 -20.08 -109.06 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -20.08 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.228

(partial model without unconserved sides chains): PDB file : Tito_4QT9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QT9-query.scw PDB file : Tito_Scwrl_4QT9.pdb: